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Document 3321
DOCN M94A3321
TI Unintegrated HIV-specific DNA in plasma of infected individuals.
DT 9412
AU Nandi J; Bhavalkar V; Kamat H; Banerjee K; National Inst. of Virology,
Pune, India.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):102 (abstract no. PA0027). Unique
Identifier : AIDSLINE ICA10/94369254
AB OBJECTIVE: The study was undertaken to detect HIV-specific DNA in plasma
of infected individuals and compare it with the other nucleic acid
markers of the virus i.e. HIV-specific DNA in PBL as the provirus and
HIV-specific RNA, in the plasma of infected persons. METHODS: Blood
samples were collected from asymptomatic, seropositive persons with risk
behaviour of HIV-transmission, patients of AIDS and voluntary donors
with no risk behaviour as control. All samples were first screened
serologically and then subjected to PCR using HIV-specific primers from
pol region. RESULTS: The study shows presence of HIV-specific DNA in the
plasma of asymptomatic, seropositive persons as well as in plasma of
AIDS patients. At the same time point, the PCR signals with DNA
extracted from PBL (representing integrated proviral DNA) were either
weak or negative. The PCR signals obtained when RNA was extracted from
plasma of the same individuals and subjected to RT-PCR were more
consistent than those of PBL-DNA-PCR. DNA extracted from plasma of AIDS
patients gave positive signals with 32p labeled full length cloned
HIV-DNA. DISCUSSION & CONCLUSION: The above results suggest that HIV
probably also replicates through an alternative pathway of unintegrated
DNA (J. Nandi & K. Banerjee, Med. Hyp. 40: 1-7, 1993). The plasma
HIV-DNA can be used in early diagnosis and monitoring therapy in HIV
infection.
DE Biological Markers/BLOOD DNA Primers/GENETICS DNA,
Viral/*BLOOD/GENETICS Genes, pol Human HIV/GENETICS/*ISOLATION &
PURIF/PHYSIOLOGY HIV Infections/DIAGNOSIS/*MICROBIOLOGY Polymerase
Chain Reaction Proviruses/GENETICS/ISOLATION & PURIF RNA,
Viral/BLOOD/GENETICS Virus Integration Virus Replication MEETING
ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).